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Genechem control lentiviral vectors
Control Lentiviral Vectors, supplied by Genechem, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+vector/control+negative/pmc13018931-67-13-19
Average 86 stars, based on 1 article reviews
control lentiviral vectors - by Bioz Stars, 2026-10
86/100 stars

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Related Articles

Recombinant:

Article Title: NDUFA5 deficiency promotes renal inflammation in diabetic nephropathy via mitochondrial ROS signaling
Article Snippet: .. Recombinant AAV9 expressing mouse Ndufa5 ( NM_026614 ) was constructed using the CV232 vector (serotype 9, EGFP reporter) by GeneChem (Order #GOSV0 378247). ..

Article Title: Macrophage PSRC1 attenuates atherosclerosis via extracellular vesicle-mediated MBD2 delivery to induce PCSK9 promoter methylation in hepatocytes.
Article Snippet: To evaluate the transcriptional activation activity of PCSK9, a dual-luciferase reporter assay was conducted using the Promega kit according to established protocols. .. For in vitro overexpression, a recombinant adenovirus encoding the mouse Psrc1 gene (Ad-PSRC1) and an empty control vector (Ad-NC) were synthesized by Genechem. ..

Article Title: Targeting NFAT5 attenuates high sodium-induced vascular aging by re-sensitizing senescent smooth muscle cells to apoptosis.
Article Snippet: Chronic high sodium intake precipitates vascular aging; however, the molecular signaling remain poorly defined.. Nuclear factor of activated T cells 5 (NFAT5) is a central regulator of the cellular osmotic stress response; however, its specific role in modulating vascular senescence signaling remains unknown.. Here, we investigated whether NFAT5 signaling mediates high‐sodium-induced vascular aging and evaluated the therapeutic potential of the specific NFAT5 inhibitor KRN5.

Expressing:

Article Title: NDUFA5 deficiency promotes renal inflammation in diabetic nephropathy via mitochondrial ROS signaling
Article Snippet: .. Recombinant AAV9 expressing mouse Ndufa5 ( NM_026614 ) was constructed using the CV232 vector (serotype 9, EGFP reporter) by GeneChem (Order #GOSV0 378247). ..

Construct:

Article Title: NDUFA5 deficiency promotes renal inflammation in diabetic nephropathy via mitochondrial ROS signaling
Article Snippet: .. Recombinant AAV9 expressing mouse Ndufa5 ( NM_026614 ) was constructed using the CV232 vector (serotype 9, EGFP reporter) by GeneChem (Order #GOSV0 378247). ..

Article Title: Enhanced ITGA3 expression induced by helicobacter pylori infection facilitates gastric cancer progression via NF-κB and Smad4.
Article Snippet: 1 Research Center of Basic Medicine, Jinan Central Hospital, Shandong First Medical University, Jinan 250013, People’s Republic of China 2 Experimental Center, Shandong University of Traditional Chinese Medicine, Jinan 250355, People’s Republic of China 3 Department of Clinical Laboratory, Qingdao Chest Hospital, Qingdao 266003, People’s Republic of China 4 Research Center of Basic Medicine, Jinan Central Hospital, Shandong University, Jinan 250013, People’s Republic of China Abstract Helicobacter pylori (H. pylori) infection is a key risk factor in the onset and progression of gastric cancer (GC).. The integrin subunit alpha 3 (ITGA3) is frequently upregulated in various malignancies and may influence cell proliferation and metastasis under specific conditions.. However, the mechanisms that govern ITGA3 activation and its role in the inflammation-driven transformation of GC remain poorly understood.

Article Title: Targeting NFAT5 attenuates high sodium-induced vascular aging by re-sensitizing senescent smooth muscle cells to apoptosis.
Article Snippet: Chronic high sodium intake precipitates vascular aging; however, the molecular signaling remain poorly defined.. Nuclear factor of activated T cells 5 (NFAT5) is a central regulator of the cellular osmotic stress response; however, its specific role in modulating vascular senescence signaling remains unknown.. Here, we investigated whether NFAT5 signaling mediates high‐sodium-induced vascular aging and evaluated the therapeutic potential of the specific NFAT5 inhibitor KRN5.

shRNA:

Article Title: IL-36γ Drives Th9 cell Differentiation via IκBζ to Sustain Antitumor Immunity in Adoptive Cell Therapy
Article Snippet: Concentrations of IL-9 and IL-10 in the culture supernatant were determined by ELISA kits (Thermo Fisher Scientific), according to the manufacturers’ instructions. .. ShRNA knockdown lentiviral particles targeting mouse Batf3 (sequence: 5’-GAAAGTTCGAAGGAGAGAGAA-3’) and non-targeting control shRNA (sequence: 5’-TTCTCCGAACGTGTCACGT-3’) were synthesized by GENECHEM (Shanghai, China) using the GV644 lentiviral vector (pRRLSIN-cPPT-U6-shRNA-SFFV-EGFP-SV40-puromycin). ..

Article Title: FTO Deficiency Inhibits Vascular Endothelial Cell Apoptosis and Improved Mitophagy in Obesity-Related Hypertension by Enhancing NDRG1 m6A Methylation.
Article Snippet: Vascular endothelial dysfunction contributes to obesity-related hypertension, yet its mechanisms remain unclear.. This study examined the role of N6-methyladenosine (m6A) in this process using in vivo and in vitro models.. C57BL/6J mice fed a highfat diet (HFD) developed obesity-related hypertension, while oxidized low-density lipoprotein (ox-LDL)-treated human aortic endothelial cells (HAECs) simulated endothelial injury.

Knockdown:

Article Title: IL-36γ Drives Th9 cell Differentiation via IκBζ to Sustain Antitumor Immunity in Adoptive Cell Therapy
Article Snippet: Concentrations of IL-9 and IL-10 in the culture supernatant were determined by ELISA kits (Thermo Fisher Scientific), according to the manufacturers’ instructions. .. ShRNA knockdown lentiviral particles targeting mouse Batf3 (sequence: 5’-GAAAGTTCGAAGGAGAGAGAA-3’) and non-targeting control shRNA (sequence: 5’-TTCTCCGAACGTGTCACGT-3’) were synthesized by GENECHEM (Shanghai, China) using the GV644 lentiviral vector (pRRLSIN-cPPT-U6-shRNA-SFFV-EGFP-SV40-puromycin). ..

Sequencing:

Article Title: IL-36γ Drives Th9 cell Differentiation via IκBζ to Sustain Antitumor Immunity in Adoptive Cell Therapy
Article Snippet: Concentrations of IL-9 and IL-10 in the culture supernatant were determined by ELISA kits (Thermo Fisher Scientific), according to the manufacturers’ instructions. .. ShRNA knockdown lentiviral particles targeting mouse Batf3 (sequence: 5’-GAAAGTTCGAAGGAGAGAGAA-3’) and non-targeting control shRNA (sequence: 5’-TTCTCCGAACGTGTCACGT-3’) were synthesized by GENECHEM (Shanghai, China) using the GV644 lentiviral vector (pRRLSIN-cPPT-U6-shRNA-SFFV-EGFP-SV40-puromycin). ..

Control:

Article Title: IL-36γ Drives Th9 cell Differentiation via IκBζ to Sustain Antitumor Immunity in Adoptive Cell Therapy
Article Snippet: Concentrations of IL-9 and IL-10 in the culture supernatant were determined by ELISA kits (Thermo Fisher Scientific), according to the manufacturers’ instructions. .. ShRNA knockdown lentiviral particles targeting mouse Batf3 (sequence: 5’-GAAAGTTCGAAGGAGAGAGAA-3’) and non-targeting control shRNA (sequence: 5’-TTCTCCGAACGTGTCACGT-3’) were synthesized by GENECHEM (Shanghai, China) using the GV644 lentiviral vector (pRRLSIN-cPPT-U6-shRNA-SFFV-EGFP-SV40-puromycin). ..

Article Title: Macrophage PSRC1 attenuates atherosclerosis via extracellular vesicle-mediated MBD2 delivery to induce PCSK9 promoter methylation in hepatocytes.
Article Snippet: To evaluate the transcriptional activation activity of PCSK9, a dual-luciferase reporter assay was conducted using the Promega kit according to established protocols. .. For in vitro overexpression, a recombinant adenovirus encoding the mouse Psrc1 gene (Ad-PSRC1) and an empty control vector (Ad-NC) were synthesized by Genechem. ..

Article Title: Enhanced ITGA3 expression induced by helicobacter pylori infection facilitates gastric cancer progression via NF-κB and Smad4.
Article Snippet: 1 Research Center of Basic Medicine, Jinan Central Hospital, Shandong First Medical University, Jinan 250013, People’s Republic of China 2 Experimental Center, Shandong University of Traditional Chinese Medicine, Jinan 250355, People’s Republic of China 3 Department of Clinical Laboratory, Qingdao Chest Hospital, Qingdao 266003, People’s Republic of China 4 Research Center of Basic Medicine, Jinan Central Hospital, Shandong University, Jinan 250013, People’s Republic of China Abstract Helicobacter pylori (H. pylori) infection is a key risk factor in the onset and progression of gastric cancer (GC).. The integrin subunit alpha 3 (ITGA3) is frequently upregulated in various malignancies and may influence cell proliferation and metastasis under specific conditions.. However, the mechanisms that govern ITGA3 activation and its role in the inflammation-driven transformation of GC remain poorly understood.

Article Title: Targeting NFAT5 attenuates high sodium-induced vascular aging by re-sensitizing senescent smooth muscle cells to apoptosis.
Article Snippet: Chronic high sodium intake precipitates vascular aging; however, the molecular signaling remain poorly defined.. Nuclear factor of activated T cells 5 (NFAT5) is a central regulator of the cellular osmotic stress response; however, its specific role in modulating vascular senescence signaling remains unknown.. Here, we investigated whether NFAT5 signaling mediates high‐sodium-induced vascular aging and evaluated the therapeutic potential of the specific NFAT5 inhibitor KRN5.

Article Title: Piezo1 mediates hypoxia-induced endometriosis fibrosis via the mtDNA -dependent cGAS-STING pathway.
Article Snippet: .. Lentiviral particles for Piezo1‐targeting shRNAs (shRNA1 and shRNA2) and a non‐targeting control were generated using the GV493 vector (obtained from Shanghai GeneChem Co.,Ltd), with titers exceeding 1 × 107 TU/mL. ..

Synthesized:

Article Title: IL-36γ Drives Th9 cell Differentiation via IκBζ to Sustain Antitumor Immunity in Adoptive Cell Therapy
Article Snippet: Concentrations of IL-9 and IL-10 in the culture supernatant were determined by ELISA kits (Thermo Fisher Scientific), according to the manufacturers’ instructions. .. ShRNA knockdown lentiviral particles targeting mouse Batf3 (sequence: 5’-GAAAGTTCGAAGGAGAGAGAA-3’) and non-targeting control shRNA (sequence: 5’-TTCTCCGAACGTGTCACGT-3’) were synthesized by GENECHEM (Shanghai, China) using the GV644 lentiviral vector (pRRLSIN-cPPT-U6-shRNA-SFFV-EGFP-SV40-puromycin). ..

Article Title: Macrophage PSRC1 attenuates atherosclerosis via extracellular vesicle-mediated MBD2 delivery to induce PCSK9 promoter methylation in hepatocytes.
Article Snippet: To evaluate the transcriptional activation activity of PCSK9, a dual-luciferase reporter assay was conducted using the Promega kit according to established protocols. .. For in vitro overexpression, a recombinant adenovirus encoding the mouse Psrc1 gene (Ad-PSRC1) and an empty control vector (Ad-NC) were synthesized by Genechem. ..

In Vitro:

Article Title: Macrophage PSRC1 attenuates atherosclerosis via extracellular vesicle-mediated MBD2 delivery to induce PCSK9 promoter methylation in hepatocytes.
Article Snippet: To evaluate the transcriptional activation activity of PCSK9, a dual-luciferase reporter assay was conducted using the Promega kit according to established protocols. .. For in vitro overexpression, a recombinant adenovirus encoding the mouse Psrc1 gene (Ad-PSRC1) and an empty control vector (Ad-NC) were synthesized by Genechem. ..

Over Expression:

Article Title: Macrophage PSRC1 attenuates atherosclerosis via extracellular vesicle-mediated MBD2 delivery to induce PCSK9 promoter methylation in hepatocytes.
Article Snippet: To evaluate the transcriptional activation activity of PCSK9, a dual-luciferase reporter assay was conducted using the Promega kit according to established protocols. .. For in vitro overexpression, a recombinant adenovirus encoding the mouse Psrc1 gene (Ad-PSRC1) and an empty control vector (Ad-NC) were synthesized by Genechem. ..

Article Title: FTO Deficiency Inhibits Vascular Endothelial Cell Apoptosis and Improved Mitophagy in Obesity-Related Hypertension by Enhancing NDRG1 m6A Methylation.
Article Snippet: Vascular endothelial dysfunction contributes to obesity-related hypertension, yet its mechanisms remain unclear.. This study examined the role of N6-methyladenosine (m6A) in this process using in vivo and in vitro models.. C57BL/6J mice fed a highfat diet (HFD) developed obesity-related hypertension, while oxidized low-density lipoprotein (ox-LDL)-treated human aortic endothelial cells (HAECs) simulated endothelial injury.

Negative Control:

Article Title: FTO Deficiency Inhibits Vascular Endothelial Cell Apoptosis and Improved Mitophagy in Obesity-Related Hypertension by Enhancing NDRG1 m6A Methylation.
Article Snippet: Vascular endothelial dysfunction contributes to obesity-related hypertension, yet its mechanisms remain unclear.. This study examined the role of N6-methyladenosine (m6A) in this process using in vivo and in vitro models.. C57BL/6J mice fed a highfat diet (HFD) developed obesity-related hypertension, while oxidized low-density lipoprotein (ox-LDL)-treated human aortic endothelial cells (HAECs) simulated endothelial injury.

Article Title: TRIM71 suppresses cervical cancer progression by inhibiting Nectin4-mediated Wnt/β-catenin signaling.
Article Snippet: .. cell lines Lentiviral particles encoding full-length human TRIM71 and the corresponding negative control vector were purchased from GeneChem (Shanghai, China). ..

Generated:

Article Title: Enhanced ITGA3 expression induced by helicobacter pylori infection facilitates gastric cancer progression via NF-κB and Smad4.
Article Snippet: 1 Research Center of Basic Medicine, Jinan Central Hospital, Shandong First Medical University, Jinan 250013, People’s Republic of China 2 Experimental Center, Shandong University of Traditional Chinese Medicine, Jinan 250355, People’s Republic of China 3 Department of Clinical Laboratory, Qingdao Chest Hospital, Qingdao 266003, People’s Republic of China 4 Research Center of Basic Medicine, Jinan Central Hospital, Shandong University, Jinan 250013, People’s Republic of China Abstract Helicobacter pylori (H. pylori) infection is a key risk factor in the onset and progression of gastric cancer (GC).. The integrin subunit alpha 3 (ITGA3) is frequently upregulated in various malignancies and may influence cell proliferation and metastasis under specific conditions.. However, the mechanisms that govern ITGA3 activation and its role in the inflammation-driven transformation of GC remain poorly understood.

Article Title: Piezo1 mediates hypoxia-induced endometriosis fibrosis via the mtDNA -dependent cGAS-STING pathway.
Article Snippet: .. Lentiviral particles for Piezo1‐targeting shRNAs (shRNA1 and shRNA2) and a non‐targeting control were generated using the GV493 vector (obtained from Shanghai GeneChem Co.,Ltd), with titers exceeding 1 × 107 TU/mL. ..

Plasmid Preparation:

Article Title: Enhanced ITGA3 expression induced by helicobacter pylori infection facilitates gastric cancer progression via NF-κB and Smad4.
Article Snippet: 1 Research Center of Basic Medicine, Jinan Central Hospital, Shandong First Medical University, Jinan 250013, People’s Republic of China 2 Experimental Center, Shandong University of Traditional Chinese Medicine, Jinan 250355, People’s Republic of China 3 Department of Clinical Laboratory, Qingdao Chest Hospital, Qingdao 266003, People’s Republic of China 4 Research Center of Basic Medicine, Jinan Central Hospital, Shandong University, Jinan 250013, People’s Republic of China Abstract Helicobacter pylori (H. pylori) infection is a key risk factor in the onset and progression of gastric cancer (GC).. The integrin subunit alpha 3 (ITGA3) is frequently upregulated in various malignancies and may influence cell proliferation and metastasis under specific conditions.. However, the mechanisms that govern ITGA3 activation and its role in the inflammation-driven transformation of GC remain poorly understood.



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<t>TCF7</t> regulates pro−caspase−8 expression in T lymphocytes and is significantly reduced in COPD. ( A ) Immunofluorescence co−staining of control human lung tissue displaying separate channels for DAPI (blue), caspase−8 (green), TCF7 (red), and the merged image. Scale bar is 50 μm. ( B ) Immunofluorescence co−staining of COPD human lung tissue displaying separate channels for DAPI (blue), caspase−8 (green), TCF7 (red), and the merged image. Note the marked reduction in both TCF7 and caspase−8 signals compared to the control. Scale bar is 50 μm. ( C ) Representative Western blot images of TCF7 (50 kDa), pro−caspase−8 (55 kDa), and internal control β−tubulin (55 kDa) in wild type (WT) and TCF7 knockout (KO) Jurkat T cells. ( D ) Quantitative densitometric analysis of TCF7 protein levels comparing WT and KO groups. ( E ) Quantitative densitometric analysis of pro−caspase−8 protein levels comparing WT and KO groups. ( F ) Representative Western blot images of TCF7 and β−tubulin in primary T lymphocytes isolated from the peripheral blood of healthy donors (Control) and patients with COPD (Model). ( G ) Quantitative densitometric analysis of TCF7 protein levels in human primary T lymphocytes. ( H ) Representative Western blot images of TCF7 and β−tubulin protein levels in Jurkat T cells across four experimental conditions including Control, <t>shRNA,</t> shRNA plus TCF7 Rescue construct, and shRNA plus Empty Vector. ( I ) Quantitative densitometric analysis of TCF7 protein levels across the four experimental rescue groups. ( J ) Representative Western blot images of pro−caspase−8 and β−tubulin protein levels across the same four experimental conditions in Jurkat T cells. ( K ) Quantitative densitometric analysis of pro−caspase−8 protein levels across the four experimental rescue groups. Data in the bar charts are presented as mean ± SD ( n = 4 for primary human cells, n = 3 for cell line experiments). Statistical significance was assessed using Student’s t test with Welch’s correction where appropriate (* p < 0.05, *** p < 0.001, ns indicates not significant).
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<t>TCF7</t> regulates pro−caspase−8 expression in T lymphocytes and is significantly reduced in COPD. ( A ) Immunofluorescence co−staining of control human lung tissue displaying separate channels for DAPI (blue), caspase−8 (green), TCF7 (red), and the merged image. Scale bar is 50 μm. ( B ) Immunofluorescence co−staining of COPD human lung tissue displaying separate channels for DAPI (blue), caspase−8 (green), TCF7 (red), and the merged image. Note the marked reduction in both TCF7 and caspase−8 signals compared to the control. Scale bar is 50 μm. ( C ) Representative Western blot images of TCF7 (50 kDa), pro−caspase−8 (55 kDa), and internal control β−tubulin (55 kDa) in wild type (WT) and TCF7 knockout (KO) Jurkat T cells. ( D ) Quantitative densitometric analysis of TCF7 protein levels comparing WT and KO groups. ( E ) Quantitative densitometric analysis of pro−caspase−8 protein levels comparing WT and KO groups. ( F ) Representative Western blot images of TCF7 and β−tubulin in primary T lymphocytes isolated from the peripheral blood of healthy donors (Control) and patients with COPD (Model). ( G ) Quantitative densitometric analysis of TCF7 protein levels in human primary T lymphocytes. ( H ) Representative Western blot images of TCF7 and β−tubulin protein levels in Jurkat T cells across four experimental conditions including Control, <t>shRNA,</t> shRNA plus TCF7 Rescue construct, and shRNA plus Empty Vector. ( I ) Quantitative densitometric analysis of TCF7 protein levels across the four experimental rescue groups. ( J ) Representative Western blot images of pro−caspase−8 and β−tubulin protein levels across the same four experimental conditions in Jurkat T cells. ( K ) Quantitative densitometric analysis of pro−caspase−8 protein levels across the four experimental rescue groups. Data in the bar charts are presented as mean ± SD ( n = 4 for primary human cells, n = 3 for cell line experiments). Statistical significance was assessed using Student’s t test with Welch’s correction where appropriate (* p < 0.05, *** p < 0.001, ns indicates not significant).
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<t>TCF7</t> regulates pro−caspase−8 expression in T lymphocytes and is significantly reduced in COPD. ( A ) Immunofluorescence co−staining of control human lung tissue displaying separate channels for DAPI (blue), caspase−8 (green), TCF7 (red), and the merged image. Scale bar is 50 μm. ( B ) Immunofluorescence co−staining of COPD human lung tissue displaying separate channels for DAPI (blue), caspase−8 (green), TCF7 (red), and the merged image. Note the marked reduction in both TCF7 and caspase−8 signals compared to the control. Scale bar is 50 μm. ( C ) Representative Western blot images of TCF7 (50 kDa), pro−caspase−8 (55 kDa), and internal control β−tubulin (55 kDa) in wild type (WT) and TCF7 knockout (KO) Jurkat T cells. ( D ) Quantitative densitometric analysis of TCF7 protein levels comparing WT and KO groups. ( E ) Quantitative densitometric analysis of pro−caspase−8 protein levels comparing WT and KO groups. ( F ) Representative Western blot images of TCF7 and β−tubulin in primary T lymphocytes isolated from the peripheral blood of healthy donors (Control) and patients with COPD (Model). ( G ) Quantitative densitometric analysis of TCF7 protein levels in human primary T lymphocytes. ( H ) Representative Western blot images of TCF7 and β−tubulin protein levels in Jurkat T cells across four experimental conditions including Control, <t>shRNA,</t> shRNA plus TCF7 Rescue construct, and shRNA plus Empty Vector. ( I ) Quantitative densitometric analysis of TCF7 protein levels across the four experimental rescue groups. ( J ) Representative Western blot images of pro−caspase−8 and β−tubulin protein levels across the same four experimental conditions in Jurkat T cells. ( K ) Quantitative densitometric analysis of pro−caspase−8 protein levels across the four experimental rescue groups. Data in the bar charts are presented as mean ± SD ( n = 4 for primary human cells, n = 3 for cell line experiments). Statistical significance was assessed using Student’s t test with Welch’s correction where appropriate (* p < 0.05, *** p < 0.001, ns indicates not significant).
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Image Search Results


TCF7 regulates pro−caspase−8 expression in T lymphocytes and is significantly reduced in COPD. ( A ) Immunofluorescence co−staining of control human lung tissue displaying separate channels for DAPI (blue), caspase−8 (green), TCF7 (red), and the merged image. Scale bar is 50 μm. ( B ) Immunofluorescence co−staining of COPD human lung tissue displaying separate channels for DAPI (blue), caspase−8 (green), TCF7 (red), and the merged image. Note the marked reduction in both TCF7 and caspase−8 signals compared to the control. Scale bar is 50 μm. ( C ) Representative Western blot images of TCF7 (50 kDa), pro−caspase−8 (55 kDa), and internal control β−tubulin (55 kDa) in wild type (WT) and TCF7 knockout (KO) Jurkat T cells. ( D ) Quantitative densitometric analysis of TCF7 protein levels comparing WT and KO groups. ( E ) Quantitative densitometric analysis of pro−caspase−8 protein levels comparing WT and KO groups. ( F ) Representative Western blot images of TCF7 and β−tubulin in primary T lymphocytes isolated from the peripheral blood of healthy donors (Control) and patients with COPD (Model). ( G ) Quantitative densitometric analysis of TCF7 protein levels in human primary T lymphocytes. ( H ) Representative Western blot images of TCF7 and β−tubulin protein levels in Jurkat T cells across four experimental conditions including Control, shRNA, shRNA plus TCF7 Rescue construct, and shRNA plus Empty Vector. ( I ) Quantitative densitometric analysis of TCF7 protein levels across the four experimental rescue groups. ( J ) Representative Western blot images of pro−caspase−8 and β−tubulin protein levels across the same four experimental conditions in Jurkat T cells. ( K ) Quantitative densitometric analysis of pro−caspase−8 protein levels across the four experimental rescue groups. Data in the bar charts are presented as mean ± SD ( n = 4 for primary human cells, n = 3 for cell line experiments). Statistical significance was assessed using Student’s t test with Welch’s correction where appropriate (* p < 0.05, *** p < 0.001, ns indicates not significant).

Journal: International Journal of Molecular Sciences

Article Title: Unfolding Immune Dysregulation in COPD: Identification of a Three-Gene Signature and Functional Validation of TCF7 in Human Lung Tissue and T Lymphocytes

doi: 10.3390/ijms27104231

Figure Lengend Snippet: TCF7 regulates pro−caspase−8 expression in T lymphocytes and is significantly reduced in COPD. ( A ) Immunofluorescence co−staining of control human lung tissue displaying separate channels for DAPI (blue), caspase−8 (green), TCF7 (red), and the merged image. Scale bar is 50 μm. ( B ) Immunofluorescence co−staining of COPD human lung tissue displaying separate channels for DAPI (blue), caspase−8 (green), TCF7 (red), and the merged image. Note the marked reduction in both TCF7 and caspase−8 signals compared to the control. Scale bar is 50 μm. ( C ) Representative Western blot images of TCF7 (50 kDa), pro−caspase−8 (55 kDa), and internal control β−tubulin (55 kDa) in wild type (WT) and TCF7 knockout (KO) Jurkat T cells. ( D ) Quantitative densitometric analysis of TCF7 protein levels comparing WT and KO groups. ( E ) Quantitative densitometric analysis of pro−caspase−8 protein levels comparing WT and KO groups. ( F ) Representative Western blot images of TCF7 and β−tubulin in primary T lymphocytes isolated from the peripheral blood of healthy donors (Control) and patients with COPD (Model). ( G ) Quantitative densitometric analysis of TCF7 protein levels in human primary T lymphocytes. ( H ) Representative Western blot images of TCF7 and β−tubulin protein levels in Jurkat T cells across four experimental conditions including Control, shRNA, shRNA plus TCF7 Rescue construct, and shRNA plus Empty Vector. ( I ) Quantitative densitometric analysis of TCF7 protein levels across the four experimental rescue groups. ( J ) Representative Western blot images of pro−caspase−8 and β−tubulin protein levels across the same four experimental conditions in Jurkat T cells. ( K ) Quantitative densitometric analysis of pro−caspase−8 protein levels across the four experimental rescue groups. Data in the bar charts are presented as mean ± SD ( n = 4 for primary human cells, n = 3 for cell line experiments). Statistical significance was assessed using Student’s t test with Welch’s correction where appropriate (* p < 0.05, *** p < 0.001, ns indicates not significant).

Article Snippet: Short hairpin RNA targeting human TCF7 (shRNA) and a scramble control shRNA were purchased from OriGene with Cat.No.TR30004.

Techniques: Expressing, Immunofluorescence, Staining, Control, Western Blot, Knock-Out, Isolation, shRNA, Construct, Plasmid Preparation